| P-BTK

Bruton's tyrosine kinase (abbreviated as Btk or BTK), also known as tyrosine-protein kinase BTK, is a tyrosine kinase encoded by the BTK gene that plays a critical role in B-cell development. BTK consists of five distinct protein-interaction domains. These include an N-terminal pleckstrin homology (PH) domain, a proline-rich Tec homology (TH) domain, Src homology (SH) domains SH2 and SH3, and a catalytic kinase domain responsible for its enzymatic activity.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.1	1.4	0.88	0.58	0.37	0.27	0.2	0.07
Corrected OD	2.03	1.33	0.81	0.51	0.3	0.2	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human P-BTK spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Culture Supernates(n=8)	95-108	104

 

| Linearity

Samples were spiked with high concentrations of Human P-BTK and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	87-96	90-110
	Average (%)	92	96
1 : 4	Range (%)	87-108	105-115
	Average (%)	98	108